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Multiple primer pairs polymerase chain reaction for the detection of human papillomavirus types
M M Anceschi1, C Falcinelli, M Pieretti
1Istituto di Ginecologia ed Ostetricia, Policlinico Monteluce, Perugia, Italy.
Journal of Virological Methods
|April 1, 1990
Abstract:
A polymerase chain reaction (PCR) based on the use of multiple primers enabling the simultaneous detection of HPV-6b, -11, -16 and -18 in a single tube reaction was developed and validated on cervico-vaginal specimens, including tissues embedded in paraffin. This PCR setting proved to be specific and sensitive, allowing the detection of as few as 200 viral particles per specimen.