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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:

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Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

Mass spectrometry accelerates membrane protein analysis.

Jeffrey N Savas1, Benjamin D Stein, Christine C Wu

  • 1Department of Chemical Physiology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, USA.

Trends in Biochemical Sciences
|May 28, 2011
PubMed
Summary
This summary is machine-generated.

Proteomic mass spectrometry advances the study of cellular membranes and their proteins. This research highlights discovery-based pipelines for a complete membrane proteome, aiding cellular homeostasis research.

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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

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Last Updated: Jun 1, 2026

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

Area of Science:

  • Cellular biology
  • Biochemistry
  • Proteomics

Background:

  • Cellular membranes, composed of proteins and lipids, are vital for cellular integrity and homeostasis.
  • Membrane proteins execute critical functions, including transport, signaling, and enzymatic activity.
  • Understanding the plasma membrane (PM) proteome is crucial for cellular function research.

Purpose of the Study:

  • To discuss recent advancements in proteomic mass spectrometry for membrane protein analysis.
  • To highlight the success of discovery-based proteomic pipelines in establishing a complete membrane proteome.
  • To underscore the importance of PM proteome research for understanding cellular mechanisms.

Main Methods:

  • Utilizing advanced proteomic mass spectrometry techniques.
  • Employing discovery-based proteomic pipelines.
  • Analyzing membrane protein topology and complexes.

Main Results:

  • Substantial progress in determining the plasma membrane (PM) proteome.
  • Improved resolution of membrane protein topology.
  • Identification of numerous receptor protein complexes and ligand-receptor pairs.
  • Elucidation of signaling networks originating at the PM.

Conclusions:

  • Proteomic mass spectrometry has accelerated the discovery of membrane proteins.
  • Discovery-based pipelines are effective for comprehensive membrane proteome characterization.
  • Continued research on the PM proteome will enhance understanding of cellular integrity and homeostasis.