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Updated: Jun 1, 2026
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
A simple and sensitive fluorescence quenching method for the determination of H(2)O(2) using Rhodamine B and
Zhiliang Jiang1, Li Kun, Huixiang Ouyang
1Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Engineering and Protection and Assessment, Guangxi Normal University, Guilin 541004, China. zljiang@mailbox.gxnu.edu.cn
Abstract:
In pH 1.99 sodium acetate-HCl buffer solutions at 60 °C, Rhodamine B exhibited a strong fluorescence peak at 584 nm using an excitation wavelength of 548 nm. The fluorescence quenching occurred when Fe(3)O(4) nanoparticles catalyzed H(2)O(2) oxidation of Rhodamine B. Under the chosen conditions, the fluorescence intensity at 584 nm decreased when the concentration of H(2)O(2) increased. The fluorescence quenching intensity is linear with the concentration of H(2)O(2) in the range of 10-200 nmol/L. Thus, a new and simple and sensitive nanocatalytic fluorescence method was proposed for the determination of H(2)O(2) in synthetic sample, with satisfactory results.
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