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High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

Published on: July 5, 2024

Stem cell migration: a zebrafish model.

Pulin Li¹, Leonard I Zon

¹Howard Hughes Medical Institute, Harvard Stem Cell Institute, Children’s Hospital Boston, Harvard Medical School, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 28, 2011

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Zebrafish offer superior in vivo imaging for studying stem cell migration and fate. Protocols are provided for tracking fluorescently labeled hematopoietic stem cells (HSC) in zebrafish embryos and adults.

Area of Science:

Developmental Biology
Stem Cell Biology
Comparative Medicine

Background:

Zebrafish (Danio rerio) possess conserved tissues and organs similar to mammals, making them suitable models for stem cell research.
Their external fertilization, embryonic transparency, and the availability of transparent mutants like 'casper' facilitate in vivo imaging.
Zebrafish are increasingly utilized for studying hematopoietic stem cells (HSC) and their migration patterns.

Purpose of the Study:

To outline protocols for in vivo imaging of stem cell migration and fate tracing in zebrafish.
To demonstrate the application of these techniques using hematopoietic stem cells (HSC) as a model.
To highlight the utility of zebrafish, particularly the 'casper' mutant, for advanced stem cell research.

Main Methods:

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High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

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Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Published on: April 29, 2016

Utilizing the transparent zebrafish model, including the 'casper' mutant, for enhanced in vivo imaging.
Employing fluorescence labeling techniques to track stem cells.
Implementing protocols for stem cell transplantation and fate tracing in both embryonic and adult zebrafish.
Combining advanced imaging with transplantation for studying hematopoietic stem cells (HSC).

Main Results:

Demonstrated successful in vivo imaging of fluorescence-labeled stem cell migration in zebrafish.
Provided detailed protocols applicable to zebrafish embryos and adults.
Showcased the effectiveness of the 'casper' zebrafish mutant for imaging adult hematopoietic stem cells (HSC).

Conclusions:

Zebrafish provide an unparalleled platform for in vivo stem cell research due to their optical properties and genetic tractability.
The presented protocols enable detailed tracing of stem cell migration and fate in both developmental and regenerative contexts.
These techniques are adaptable for studying various stem cell types in zebrafish, advancing regenerative medicine and developmental biology.