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Luminescent trimethoprim-polyaminocarboxylate lanthanide complex conjugates for selective protein labeling and

D Rajasekhar Reddy1, Laura E Pedró Rosa, Lawrence W Miller

  • 1University of Illinois at Chicago, Department of Chemistry, 845 W. Taylor Street, MC 111, Chicago, Illinois 60607, United States.

Bioconjugate Chemistry
|May 31, 2011
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...