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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

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Updated: Jun 1, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

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Aerosol MALDI with a Reflectron Time-of-Flight Mass Spectrometer.

X Fei1, G Wei, K K Murray

  • 1Department of Chemistry, Emory University, Atlanta, Georgia 30322.

Analytical Chemistry
|May 31, 2011
PubMed
Summary
This summary is machine-generated.

Aerosol matrix-assisted laser desorption/ionization (MALDI) coupled with a reflectron time-of-flight mass spectrometer significantly enhances mass resolution. This novel approach achieved over 10-fold improvement for peptide and vitamin B12 analysis.

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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Biochemistry

Background:

  • Matrix-assisted laser desorption/ionization (MALDI) is a key technique for analyzing biomolecules.
  • Previous aerosol MALDI methods using linear time-of-flight instruments had limited mass resolution.

Purpose of the Study:

  • To improve mass resolution in aerosol MALDI by integrating a reflectron time-of-flight mass spectrometer.
  • To evaluate the performance of this enhanced system for analyzing peptides and small molecules.

Main Methods:

  • Aerosol MALDI was performed by spraying a methanol solution of matrix and analyte directly into a reflectron time-of-flight mass spectrometer.
  • Ionization was achieved using a frequency-tripled Nd:YAG laser at 355 nm.

Main Results:

  • Mass resolution exceeding 300 was achieved for bradykinin, angiotensin II, gramicidin D, and vitamin B12.
  • This represents an approximate 10-fold increase in resolution compared to previous aerosol MALDI linear time-of-flight instruments.

Conclusions:

  • Combining aerosol MALDI with a reflectron time-of-flight mass spectrometer offers a substantial improvement in mass resolution.
  • This technique provides a more sensitive and accurate method for analyzing peptides and other biomolecules.