Related Experiment Video
Updated: Jun 1, 2026

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
Published on: February 23, 2017
Classical retention mechanism in ion exchange chromatography. Theory and experiment
1Laboratoire de Chimie Technique, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
Abstract:
The classical model for ion exchange chromatography is characterized by firmly adsorbed driving ions at the surface of the stationary phase in an amount required by electroneutrality and stoichiometric ion exchange between the bulk of the eluent electrolyte and this immobilized Stern layer. Retention equations have been derived for system peaks, labeled eluent ions, and analytes in a system containing only strong electrolytes by strictly respecting this model. It is shown that the classical model described dependence of retention data on concentration and composition of the binary eluent with excellent precision, but the resulting system parameters were not self-consistent. Inconsistency of the results might be due to contributions from another retention mechanism.
Related Concept Videos
Ion-Exchange Chromatography
Ion Exchange
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Chromatographic Methods: Terminology
Principles Of Column Chromatography
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...

