Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of reaction dynamics in a trypsin-modified capillary microreactor.

Analytical chemistry·2011
Same author

Direct electrochemical detection of purine- and pyrimidine-based nucleotides with sinusoidal voltammetry.

Analytical chemistry·2011
Same author

Capillary electrophoresis.

Analytical chemistry·2010
Same author

Immobilization and detection of DNA on microfluidic chips.

Talanta·2008
Same author

Capillary gel electrophoresis with sinusoidal voltammetric detection: a strategy to allow four-"color" DNA sequencing.

Analytical chemistry·2001
Same author

Detection of native amino acids and peptides utilizing sinusoidal voltammetry.

Analytical chemistry·2000
Same journal

Machine Learning-Assisted Nanopore for Enhanced Fingerprinting Analysis of Functional Glycans.

Analytical chemistry·2026
Same journal

Correction to "Maleylpyruvic Acid-Inducible Gene Expression System and Its Application for the Development of Gentisic Acid Biosensor".

Analytical chemistry·2026
Same journal

Computer-Aided Rational Hapten Design for Broad-Spectrum Monoclonal Antibody Development against Anthraquinones and Its Application in Lateral Flow Immunoassay.

Analytical chemistry·2026
Same journal

One-Step Chemoenzymatic Labeling and Oxime-Reversible Enrichment for O-GlcNAcylation Profiling under Oxidative Stress.

Analytical chemistry·2026
Same journal

Acid/NIR Dual-Responsive Nanoplatform with AND Logic-Gated Controlled Nitric Oxide Release for Companion Theranostics of Tumors.

Analytical chemistry·2026
Same journal

Multicharged Foldable Plasma Membrane Probes for Precise Cancer Cell Discrimination and Fluorescence-Guided Surgery.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 1, 2026

Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models
10:46

Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models

Published on: May 3, 2017

Capillary biosensor for glutamate.

R J Cosford1, W G Kuhr

  • 1Department of Chemistry, University of California, Riverside, California 92521-0403.

Analytical Chemistry
|May 31, 2011
PubMed
Summary
This summary is machine-generated.

We created a novel glutamate biosensor using enzyme-immobilized capillaries for sensitive detection. This device shows rapid response and a wide linear range, making it suitable for in vivo applications.

More Related Videos

Glutamine Flux Imaging Using Genetically Encoded Sensors
10:23

Glutamine Flux Imaging Using Genetically Encoded Sensors

Published on: July 31, 2014

FIBS-enabled Noninvasive Metabolic Profiling
09:16

FIBS-enabled Noninvasive Metabolic Profiling

Published on: February 3, 2014

Related Experiment Videos

Last Updated: Jun 1, 2026

Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models
10:46

Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models

Published on: May 3, 2017

Glutamine Flux Imaging Using Genetically Encoded Sensors
10:23

Glutamine Flux Imaging Using Genetically Encoded Sensors

Published on: July 31, 2014

FIBS-enabled Noninvasive Metabolic Profiling
09:16

FIBS-enabled Noninvasive Metabolic Profiling

Published on: February 3, 2014

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Biomedical Engineering

Background:

  • Accurate glutamate detection is crucial for understanding neurological processes.
  • Existing biosensors face challenges in sensitivity and real-time monitoring.
  • Novel biosensor designs are needed for in vivo glutamate measurements.

Purpose of the Study:

  • To develop and characterize a novel glutamate biosensor.
  • To utilize enzyme immobilization within fused silica capillaries for enhanced detection.
  • To evaluate the biosensor's performance for potential in vivo applications.

Main Methods:

  • Immobilization of glutamate dehydrogenase (GDH) onto the inner surface of fused silica capillaries via biotin-avidin chemistry.
  • Detection of NADH produced by GDH enzymatic activity using laser-induced fluorescence.
  • Characterization of biosensor response time, limit of detection, linear working range, and efficiency.

Main Results:

  • A robust glutamate biosensor was successfully developed with a response time of 450 ms.
  • The biosensor demonstrated a low limit of detection (3 μM) and a linear working range of 3–300 μM.
  • Enzyme activity was retained, and efficiency correlated positively with glutamate concentration, with optimal performance at 4.5 cm/min linear velocity.

Conclusions:

  • The developed capillary-based glutamate biosensor offers high sensitivity and rapid response.
  • The biosensor's design is amenable for integration into microfluidic devices and in vivo monitoring.
  • Further development could incorporate this biosensor into capillary electrophoresis and microdialysis systems.