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[High-resolution melting: a analysis for genotyping of MDR1 C3435T in benzene-exposed workers]
Jian-shu Huang1, Xin-ju Zhang, Xiao Xu
1Department of Occupational Medicine, Fudan University, Shanghai 200540, China.
Objective:
Using high resolution melting (HRM) to analysis MDR1 C3435T in people exposed to benzene.
Methods:
Restriction fragment length polymorphism (RFLP) was utilized to detect the polymorphism of MDR1 3435 in 121 benzene-exposed workers, and the results were compared with the HRM in 10% samples and were confirmed with direct sequencing for six people in them.
Results:
By direct sequencing, consistent results of benzene-exposed workers with RFLP or HRM were got. The new high resolution melting curve analysis is more efficient, more convenient, and cheaper than RFLP.
Conclusion:
High-resolution melting analysis provides a valid approach to efficiently detect DNA genetic diagnosis, which is suitable for detect susceptible genes in occupational surveillance.
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