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Determination of glomerular filtration rate using iohexol clearance and capillary sampling
E Krutzén1, S E Bäck, P Nilsson-Ehle
1Department of Clinical Chemistry, University of Lund, Sweden.
Summary
Monitoring plasma clearance of iohexol simplifies glomerular filtration rate measurement. Using capillary blood samples for iohexol analysis provides accurate results comparable to traditional plasma sampling, enhancing convenience.
Area of Science:
- Nephrology
- Clinical Chemistry
- Pharmacokinetics
Background:
- Glomerular filtration rate (GFR) is a key indicator of kidney function.
- Plasma clearance of iohexol is a reliable method for GFR quantification.
- Current methods may be simplified for improved patient convenience.
Purpose of the Study:
- To evaluate the feasibility of using capillary blood sampling for iohexol clearance measurements.
- To determine if capillary sampling can accurately reflect plasma iohexol concentrations for GFR estimation.
Main Methods:
- Participants received an intravenous injection of iohexol.
- Plasma and capillary blood samples were collected 3-4 hours post-injection.
- Iohexol concentrations were measured using high-performance liquid chromatography (HPLC).
Main Results:
- Iohexol distribution was quantitatively confirmed in the plasma compartment.
- Plasma iohexol concentrations calculated from capillary blood showed high correlation with actual plasma concentrations (r = 0.98).
- Imprecision of iohexol determination in capillary samples was 3.5% (CV), with good agreement in clearance measurements.
Conclusions:
- Capillary blood sampling is a viable and accurate alternative to venous plasma sampling for iohexol clearance measurements.
- This simplified approach can enhance the convenience of GFR assessment.
- The method offers a reliable and precise way to quantify GFR.