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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
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High throughput label-free platform for statistical bio-molecular sensing.

Filippo G Bosco1, En-Te Hwu, Ching-Hsiu Chen

  • 1Department of Micro- and Nanotechnology, Technical University of Denmark, Lyngby, Denmark. filippo.bosco@nanotech.dtu.dk

Lab on a Chip
|May 31, 2011
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Summary

We developed a high-throughput, label-free sensor platform using cantilever sensors and DVD player components for efficient liquid sample handling and analysis. This system enables rapid, parallel detection of biomolecular binding events.

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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
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Area of Science:

  • Biosensing
  • Nanotechnology
  • Biophysics

Background:

  • High-throughput screening and online monitoring are essential for various applications, including diagnostics and environmental control.
  • Cantilever-based sensors offer potential for highly parallelized operation but lack efficient liquid sample handling and data acquisition methods.

Purpose of the Study:

  • To demonstrate a high-throughput, label-free sensor platform for analyzing liquid samples.
  • To integrate DVD player optics and mechanics for sample handling and sensor readout.
  • To leverage cantilever deflection and surface roughness for detecting biomolecular binding events.

Main Methods:

  • Utilized cantilever-based sensors for label-free detection.
  • Adapted optics and mechanics from a DVD player for automated liquid sample handling and readout of cantilever deflection and resonant frequency.
  • Measured surface roughness in conjunction with cantilever deflection.

Main Results:

  • Achieved parallel analysis of 30 liquid samples, each with 24 cantilever sensors, within minutes.
  • Demonstrated the ability to detect specific and unspecific binding events by analyzing both cantilever deflection and surface roughness.
  • Successfully detected the binding of streptavidin and antibodies.

Conclusions:

  • The developed platform provides a high-throughput, label-free sensing solution with enhanced liquid sample handling capabilities.
  • Combining cantilever deflection and surface roughness measurements offers valuable insights into binding events.
  • This approach paves the way for more efficient and parallelized biosensing applications.