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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

Oleanene triterpenes from Sedum lineare Thunb.

Xiao-Feng Niu¹, Xia Liu, Lan Pan

¹aculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China.

|June 1, 2011

Summary

This summary is machine-generated.

Researchers isolated a new olean-13(18)-ene-3,12,19-trione and two known triterpenes from Sedum linear Thunb. These compounds were tested for their inhibitory effects on tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO) production.

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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

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Published on: August 16, 2018

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Area of Science:

Natural Product Chemistry
Pharmacology
Medicinal Plant Research

Background:

Sedum linear Thunb. is a plant with potential medicinal properties.
Triterpenes are a class of compounds known for diverse biological activities.

Purpose of the Study:

To isolate and characterize compounds from Sedum linear Thunb.
To evaluate the inhibitory effects of isolated compounds on TNF-α and NO production.

Main Methods:

Phytochemical investigation of Sedum linear Thunb. using solvent extraction.
Isolation and structural elucidation of compounds using spectroscopic methods (1D, 2D NMR, HR-ESI-MS) and X-ray diffraction.
In vitro assays to measure inhibition of TNF-α and NO production.

Main Results:

One new olean-13(18)-ene-3,12,19-trione was identified.
Two known oleanene triterpenes, δ-amyrone and δ-amyrine acetate, were isolated.
The inhibitory effects of the isolated compounds on TNF-α and NO production were evaluated.

Conclusions:

The study successfully identified novel and known triterpenes from Sedum linear Thunb.
The isolated compounds demonstrated potential inhibitory effects on inflammatory mediators TNF-α and NO.
Further research is warranted to explore the therapeutic potential of these compounds.