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Redistribution of caveolae during mitosis
Emmanuel Boucrot1, Mark T Howes, Tomas Kirchhausen
1Department of Cell Biology and Immune Disease Institute, Harvard Medical School, Boston, MA 02115, USA.
Journal of Cell Science
|June 1, 2011
Summary
Caveolae, vital plasma membrane structures, dynamically change during cell division. Their redistribution from the cell surface to internal compartments during mitosis is regulated by microtubule dynamics.
Area of Science:
- Cell Biology
- Membrane Biology
- Molecular Biology
Background:
- Caveolae are specialized plasma membrane microdomains involved in cellular signaling and endocytosis.
- Caveolin-1 (Cav1) is a key structural protein of caveolae, essential for their formation and function.
Purpose of the Study:
- To investigate the dynamic behavior and localization of caveolae during the cell cycle, particularly mitosis.
- To elucidate the mechanisms regulating caveolin-1 redistribution during cell division.
Main Methods:
- Total internal reflection fluorescence (TIRF) microscopy for live-cell imaging of caveola dynamics.
- 3D fast spinning-disk confocal microscopy for extended observation periods.
- Electron microscopy for high-resolution structural analysis.
- Nocodazole treatment to study microtubule involvement.
Main Results:
- Caveolae exhibit dynamic behavior with lifetimes ranging from seconds to minutes.
- During mitosis, caveolae dynamics and localization change, leading to Cav1 redistribution from the plasma membrane to intracellular compartments.
- These changes are reversed during cytokinesis and can be mimicked by microtubule disruption.
Conclusions:
- Mitosis induces significant alterations in caveolae dynamics and Cav1 localization.
- Microtubule rearrangements during mitosis play a crucial role in mediating Cav1 relocalization.
- This study reveals precise regulation of caveola budding and recycling during cell division.
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