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TRIM28 regulate G2/M transition via histone modification and DNA damage repair during mouse oocyte meiosis

Rui-Jie Ma1, Meng-Meng Tang1, Ping-Shuang Lu1

  • 1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

Journal of Cell Science
|August 10, 2026
PubMed

Insights

Tripartite motif 28 (TRIM28) is crucial for mouse oocyte meiotic maturation, regulating the G2/M transition. Its depletion causes DNA damage and delays maturation by affecting histone modifications and DNA repair pathways.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Tripartite motif 28 (TRIM28) is a transcriptional coregulator essential for genome stability during mitosis.
  • Its role during oocyte meiotic maturation, a period of low transcriptional activity, remains largely unexplored.

Purpose of the Study:

  • To investigate the function of TRIM28 in mouse oocyte meiotic maturation.
  • To elucidate the molecular mechanisms underlying TRIM28's role in regulating the G2/M transition and genome stability.

Main Methods:

  • TRIM28 depletion using RNA interference in mouse oocytes.
  • Analysis of meiotic progression, germinal vesicle breakdown (GVBD), and first polar body (PB1) extrusion.
  • Assessment of cell cycle regulators (CDK1, cyclin B1), histone modifications (H4K12ac, H3K9me2), and DNA damage markers (γ-H2A.X, CHK1/2, RAD51).

Main Results:

  • TRIM28 is constitutively expressed and localized to the nucleus in germinal vesicle (GV)-stage oocytes.
  • TRIM28 depletion caused delayed G2/M transition, impaired GVBD, but not PB1 extrusion.
  • Depletion affected CDK1 activity, cyclin B1 levels, histone modifications, and increased DNA damage signaling, leading to GVBD failure.

Conclusions:

  • TRIM28 plays a critical role in regulating the G2/M transition during mouse oocyte meiotic maturation.
  • TRIM28 modulates histone modifications and DNA damage repair pathways to ensure proper meiotic progression.
  • TRIM28 is essential for maintaining genome stability and successful meiotic maturation in oocytes.
Keywords:
G2/M transitionMeiosisOocyteTRIM28Transcription

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