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Updated: Jun 1, 2026

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
Published on: November 11, 2018
A luminescent oxygen channeling biosensor that measures small GTPase activation
Björn Niebel1, Benjamin Weiche, Alan L Mueller
1LIMES Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, 53121 Bonn, Germany.
Abstract:
We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining activation states of diverse GTPases in various biological contexts.
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