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Related Experiment Videos

Alternative splicing dictates translational start in Epstein-Barr virus transcripts.

R P Rogers1, M Woisetschlaeger, S H Speck

  • 1Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, MA.

The EMBO Journal
|July 1, 1990
PubMed
Summary

Epstein-Barr virus (EBV) alternative splicing generates distinct EBNA mRNAs. This process controls translation initiation, ensuring efficient expression of all Epstein-Barr nuclear antigen (EBNA) genes during viral latency.

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Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Epstein-Barr virus (EBV) latency involves expression of 10 viral genes.
  • Six of these genes encode Epstein-Barr nuclear antigens (EBNAs).
  • EBNA expression relies on a long primary transcript processed via alternative splicing and polyadenylation.

Purpose of the Study:

  • To investigate the role of alternative splicing in EBNA gene expression.
  • To understand how EBNA mRNAs are generated from a common primary transcript.
  • To elucidate the mechanism controlling translation initiation for different EBNAs.

Main Methods:

  • Analysis of alternative splicing patterns in EBV latency transcripts.
  • Identification of common exons and variable splicing events.

Related Experiment Videos

  • Determination of translation initiation codon usage in different EBNA mRNAs.

    • Main Results:

    • Alternative splicing dictates the presence or absence of the translation initiation codon for EBNA 4.
    • This splicing mechanism allows for the generation of mRNAs initiating with either EBNA 4 or downstream EBNA genes.
    • Common exons from the BamHI W fragment encode the N-terminal portion of EBNA 4.

    Conclusions:

    • Alternative splicing is a key regulatory mechanism for EBNA gene expression during EBV latency.
    • This process ensures the efficient translation of all EBNA genes from a single primary transcript.
    • The findings provide insight into the complex gene regulation strategies of Epstein-Barr virus.