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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
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Manipulating corn germplasm to increase recombinant protein accumulation.

Elizabeth E Hood1, Shivakumar P Devaiah, Gina Fake

  • 1Arkansas State University, Jonesboro, AR, USA. ehood@astate.edu

Plant Biotechnology Journal
|June 2, 2011
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Transgenic maize seeds accumulate high concentrations of cellulases, lowering enzyme production costs for biofuel and biobased product development. Breeding and grain processing enhance enzyme recovery for efficient biomass conversion.

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Published on: July 23, 2013

Area of Science:

  • Biotechnology
  • Plant molecular biology
  • Enzyme engineering

Background:

  • Plants as biofactories offer a cost-effective method for industrial enzyme production.
  • High enzyme concentrations in plant tissues are crucial for economic viability in biomass conversion.
  • Cellulases are key enzymes for breaking down plant biomass into biofuels and biobased products.

Purpose of the Study:

  • To enhance the accumulation of cellulases in transgenic maize seed.
  • To reduce the production costs of cellulases for plant biomass conversion.
  • To improve the efficiency of enzyme recovery from transgenic maize.

Main Methods:

  • Genetic engineering to introduce cellulase genes into maize.
  • Conventional breeding techniques to integrate genes into elite germplasm with high oil content.
  • Grain processing methods to isolate enzyme-rich germ fractions.

Main Results:

  • Successfully enhanced cellulase accumulation in transgenic maize seeds.
  • Demonstrated increased enzyme recovery through germ isolation, improving dry weight basis.
  • Confirmed the activity of isolated cellulases on microcrystalline cellulose, releasing glucose and cellobiose.

Conclusions:

  • Transgenic maize seeds are a viable platform for high-level production of active cellulases.
  • Integrated breeding and processing strategies significantly improve enzyme yield and cost-effectiveness.
  • This approach holds substantial potential for reducing costs in biofuel and biobased product industries.