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Related Experiment Video

Updated: Jun 1, 2026

Oligopeptide Competition Assay for Phosphorylation Site Determination

Oligopeptide Competition Assay for Phosphorylation Site Determination

Published on: May 18, 2017

Ubp8 and SAGA regulate Snf1 AMP kinase activity.

Marenda A Wilson¹, Evangelia Koutelou, Calley Hirsch

¹Department of Biochemistry and Molecular Biology, Center forCancer Epigenetics, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Box 1000, Houston, Texas 77030, USA.

Molecular and Cellular Biology

|June 2, 2011

Summary

The SAGA complex modifies gene expression by regulating protein stability. This study reveals the SAGA complex deubiquitinates Snf1 kinase, impacting its activity and gene regulation.

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Published on: August 30, 2017

Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

Posttranslational modifications, such as histone acetylation and deubiquitination, are crucial for regulating gene expression.
The Saccharomyces cerevisiae (yeast) 2-MDa SAGA (Spt-Ada-Gcn5) complex is a key regulator of gene expression through histone acetylation (Gcn5) and deubiquitination (Ubp8).

Purpose of the Study:

To investigate the non-histone targets of the SAGA complex's deubiquitinating activity.
To elucidate the role of Ubp8-mediated deubiquitination of Snf1 kinase in gene expression modulation.

Main Methods:

Proteomics approach to identify SAGA complex targets.
Analysis of Snf1 kinase stability and phosphorylation state.
Assessment of Gal83 phosphorylation in UBP8 deletion mutants.

Main Results:

The SAGA complex deubiquitinates nonhistone proteins, including Snf1, an AMP-activated kinase.
Ubp8-mediated deubiquitination of Snf1 influences its stability, phosphorylation, and kinase activity.
Deletion of UBP8 leads to decreased phosphorylation of Gal83, consistent with altered Snf1 kinase function.

Conclusions:

The SAGA complex fine-tunes gene expression by modulating the posttranslational modifications of Snf1.
SAGA's role extends beyond histone modification to include regulation of key signaling kinases like Snf1.