Related Experiment Video
Updated: Jun 1, 2026
10:02
Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
Published on: June 2, 2023
Antioxidant hispidin derivatives from medicinal mushroom Inonotus hispidus
Li-feng Zan1, Jian-chun Qin, Ya-mei Zhang
1Engineering Research Centre of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China.
Chemical & Pharmaceutical Bulletin
|June 2, 2011
Summary
Two natural antioxidants, inonotusin A and B, were isolated from Inonotus hispidus. These compounds, along with others, demonstrated significant antioxidant and moderate anticancer properties.
Area of Science:
- Natural Product Chemistry
- Pharmacology
- Mycology
Background:
- Inonotus hispidus is a fungus known for its potential medicinal properties.
- Natural products are a rich source of bioactive compounds with therapeutic applications.
Purpose of the Study:
- To isolate and characterize natural antioxidants from Inonotus hispidus.
- To evaluate the antioxidant and cytotoxic activities of the isolated compounds.
Main Methods:
- Methanolic extraction of Inonotus hispidus fruit bodies.
- Structure elucidation using Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS).
- Assay of antioxidant activity using the ABTS radical cation scavenging method.
- Cytotoxicity assessment against the MCF-7 human breast carcinoma cell line.
Main Results:
- Isolation and identification of five compounds: inonotusin A (1), inonotusin B (2), (E)-4-(3,4-dihydroxyphenyl)but-3-en-2-one (3), hispidin (4), and 3,4-dihydroxybenzaldehyde (5).
- Compounds 1, 2, and 4 displayed significant ABTS radical scavenging activity.
- Inonotusin A (1) exhibited moderate cytotoxicity against MCF-7 cells with an IC50 of 19.6 µM.
Conclusions:
- Inonotus hispidus is a source of bioactive compounds with antioxidant and potential anticancer properties.
- Inonotusin A and B are novel natural antioxidants.
- Further research into the therapeutic potential of these compounds is warranted.