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Updated: Jun 1, 2026

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Published on: November 8, 2024

Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness.

E Miko¹, M Halasz, B Jericevic-Mulac

¹Department of Medical Microbiology and Immunology, Medical School, Pecs University, 12 Szigeti Street, H-7624 Pecs, Hungary.

Journal of Reproductive Immunology

|June 3, 2011

Summary

Progesterone-induced blocking factor (PIBF) regulates trophoblast invasion. PIBF expression inversely correlates with invasiveness, suggesting PIBF affects leptin signaling in pregnancy and tumors.

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Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

Published on: January 8, 2012

Area of Science:

Reproductive Biology
Molecular Endocrinology
Cancer Biology

Background:

Controlled trophoblast invasion is crucial for successful human pregnancy.
Progesterone and its induced molecule, PIBF, regulate trophoblast invasiveness.
Placental leptin also influences trophoblast invasion by affecting progesterone secretion.

Purpose of the Study:

To investigate the interaction between PIBF and leptin in regulating trophoblast invasion.
To analyze PIBF and leptin/leptin receptor expression in normal and abnormal placentae.
To determine PIBF's effect on leptin and leptin receptor expression in trophoblast cells.

Main Methods:

Immunohistochemistry for PIBF, leptin, and leptin receptor on placental tissues.
Generation of PIBF-deficient trophoblast cells using siRNA.
Western blot analysis for leptin receptor and protein array for leptin expression.

Main Results:

PIBF expression was high in normal and partial mole placentae, decreased in complete mole, and absent in choriocarcinoma.
Leptin and leptin receptor were absent in partial mole but present in complete mole and choriocarcinoma.
PIBF deficiency upregulated leptin receptor, while PIBF treatment decreased leptin expression.

Conclusions:

PIBF expression is inversely related to trophoblast invasiveness.
PIBF influences the expression of leptin and its receptor.
This interaction may play a role in placentation and tumor development.