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Updated: Jun 1, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Techno-economic implications of improved high gravity corn mash fermentation.
Arthur Kollaras1, John M Kavanagh, Geoffrey L Bell
1Microbiogen Pty Ltd., Sydney, Australia. arthur.kollaras@microbiogen.com
A novel yeast strain, Saccharomyces cerevisiae MBG3964, significantly outperforms industrial strains in high-gravity corn mash fermentation, boosting alcohol yield and viability. This high ethanol tolerance enhances corn-ethanol plant profitability through increased production and efficiency.
Area of Science:
- Biotechnology
- Industrial Microbiology
- Yeast Fermentation
Background:
- High gravity fermentation presents challenges for yeast viability and ethanol production.
- Optimizing yeast strains is crucial for improving the economic efficiency of bioethanol production.
Purpose of the Study:
- To compare the performance of Saccharomyces cerevisiae MBG3964, a high-ethanol-tolerant strain, against a leading industrial strain (Fermentis Ethanol Red).
- To evaluate the impact of this superior yeast strain on corn mash fermentation under high gravity conditions.
Main Methods:
- Comparative fermentation trials using Saccharomyces cerevisiae MBG3964 and Fermentis Ethanol Red under high gravity corn mash conditions.
- Measurement of alcohol yield, residual sugar, glycerol production, and yeast viability over 72 hours.
- Economic modeling to assess the potential profitability increase for a dry mill ethanol plant.
Main Results:
- Saccharomyces cerevisiae MBG3964 achieved higher alcohol yield (140g L(-1) vs. 126g L(-1)) and lower residual sugar (4g L(-1) vs. 32g L(-1)) compared to the industrial strain.
- MBG3964 demonstrated significantly higher viability (40% vs. 3%) after 72 hours of fermentation.
- Lower glycerol production was observed with MBG3964 (11g L(-1) vs. 12g L(-1)).
Conclusions:
- Saccharomyces cerevisiae MBG3964 offers superior performance in high gravity corn mash fermentation due to its high ethanol tolerance.
- The use of MBG3964 can lead to substantial economic benefits for corn-ethanol plants through increased alcohol yield and throughput.
- Enhanced yeast viability and fermentation efficiency contribute to improved overall profitability in the bioethanol industry.
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