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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Updated: Jun 1, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Liquid chromatography-mass spectrometry-based quantitative proteomics.

Fang Xie1, Tao Liu, Wei-Jun Qian

  • 1Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.

The Journal of Biological Chemistry
|June 3, 2011
PubMed
Summary
This summary is machine-generated.

Liquid chromatography-mass spectrometry (LC-MS) quantitative proteomics offers broad coverage, accuracy, and precision for biological studies. This review covers current LC-MS quantification methods, their pros and cons, and applications.

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Liquid chromatography-mass spectrometry (LC-MS) is a powerful technique in proteomics.
  • Its application in quantitative proteomics has grown due to advancements in technology.

Purpose of the Study:

  • To review current LC-MS-based quantification methods.
  • To discuss the advantages and limitations of these methods.
  • To highlight potential applications of LC-MS quantitative proteomics.

Main Methods:

  • Review of existing literature on LC-MS-based quantification techniques.
  • Analysis of the strengths and weaknesses of various quantification strategies.
  • Identification of current and emerging applications in biological research.

Main Results:

  • LC-MS-based quantitative proteomics provides broad proteome coverage.
  • These methods offer high accuracy and precision in quantification.
  • Various quantification strategies exist, each with specific benefits and drawbacks.

Conclusions:

  • LC-MS-based quantitative proteomics is a valuable tool for biological research.
  • Understanding the nuances of different quantification methods is crucial for experimental design.
  • The field continues to evolve, offering expanding possibilities for biological discovery.