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[The effect of high molecular weight interferon inducers (dsRNA) on virus adsorption]
Abstract:
Interrelationship between phosphorylation of plasmatic membrane proteins in brain cells and the rate of mice encephalomyocarditis virus adsorption was studied. Phosphorylation of proteins induced by dsRNA (laryphane) was most distinctly manifested in membrane fraction and cytosol of rat brain neuronal cells. Similarity of molecular mass spectra in dsRNA- and cAMP-dependent phosphorylation enabled to suggest that dsRNA activated protein kinase. An increase in the rate of plasmatic membrane proteins phosphorylation appears to correlate with inhibition of virus adsorption as shown by studies of these reactions dynamics. Under conditions of considerable increase in the rate of membrane proteins phosphorylation adsorption of virus on cells was distinctly inhibited.
Insights
Double-stranded RNA (dsRNA) triggers brain cell protein phosphorylation, which inhibits encephalomyocarditis virus adsorption. Increased phosphorylation of plasma membrane proteins correlates with reduced virus binding to cells.
Area of Science:
- Neuroscience
- Virology
- Molecular Biology
Background:
- Encephalomyocarditis virus (EMCV) infects brain cells.
- Protein phosphorylation plays a role in cellular processes.
- The effect of dsRNA on brain cell protein phosphorylation and viral adsorption is not fully understood.
Purpose of the Study:
- To investigate the relationship between brain cell plasma membrane protein phosphorylation and EMCV adsorption.
- To determine the role of dsRNA-induced phosphorylation in this process.
Main Methods:
- Studied phosphorylation of proteins in rat brain neuronal cells induced by dsRNA (laryphane).
- Analyzed protein phosphorylation in membrane and cytosol fractions.
- Compared dsRNA-dependent and cAMP-dependent phosphorylation.
- Examined the dynamics of protein phosphorylation and virus adsorption.
Main Results:
- dsRNA-induced protein phosphorylation was most prominent in neuronal cell membrane and cytosol fractions.
- dsRNA-activated protein kinase, suggested by similarities in phosphorylation spectra.
- Increased plasma membrane protein phosphorylation correlated with inhibited EMCV adsorption.
- Significant inhibition of virus adsorption was observed with heightened protein phosphorylation.
Conclusions:
- dsRNA activates protein kinase in brain cells.
- Enhanced phosphorylation of plasma membrane proteins inhibits encephalomyocarditis virus adsorption.
- This suggests a potential mechanism for cellular defense against viral entry.