Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Factors Affecting Erythropoiesis01:24

Factors Affecting Erythropoiesis

The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
Erythropoiesis01:14

Erythropoiesis

Red blood cells  (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia, and...
Erythropoiesis01:14

Erythropoiesis

Red blood cells  (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia, and...
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gel Electrophoretic Detection of Black Market ACE-031.

Drug testing and analysis·2025
Same author

Cost Minimized Immunoaffinity Purification of EPO and Its Analogs in Doping Control-A Step-by-Step Protocol for Human Urine and Blood.

Drug testing and analysis·2025
Same author

High-Frequency Rheological and Piezo-Voltage Waveform Characterization of Inkjet-Printed Polymer-Based Dopant-Source Inks.

Micromachines·2023
Same author

Electrophoretic detection of black market myostatin propeptide.

Drug testing and analysis·2022
Same author

Detection of black market follistatin 344.

Drug testing and analysis·2021
Same author

Data from a microdosed recombinant human erythropoietin administration study applying the new biotinylated clone AE7A5 antibody and a further optimized sarcosyl polyacrylamide gel electrophoresis protocol.

Drug testing and analysis·2021

Related Experiment Video

Updated: Jun 1, 2026

A Comprehensive Pipeline to Assess the Efficiency of Human Erythropoiesis In Vitro and Ex Vivo
08:53

A Comprehensive Pipeline to Assess the Efficiency of Human Erythropoiesis In Vitro and Ex Vivo

Published on: January 10, 2025

Recent developments in doping testing for erythropoietin.

Christian Reichel1

  • 1Doping Control Laboratory, AIT Seibersdorf Laboratories, Seibersdorf, Austria. christian.reichel@seibersdorf-laboratories.at

Analytical and Bioanalytical Chemistry
|June 4, 2011
PubMed
Summary

Detecting erythropoiesis-stimulating agent (ESA) doping requires evolving methods. New techniques analyze urine and blood, adapting to novel ESAs and the Athlete Biological Passport for enhanced anti-doping efforts.

More Related Videos

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
15:32

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Published on: August 5, 2011

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
14:22

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Published on: July 16, 2011

Related Experiment Videos

Last Updated: Jun 1, 2026

A Comprehensive Pipeline to Assess the Efficiency of Human Erythropoiesis In Vitro and Ex Vivo
08:53

A Comprehensive Pipeline to Assess the Efficiency of Human Erythropoiesis In Vitro and Ex Vivo

Published on: January 10, 2025

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
15:32

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Published on: August 5, 2011

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
14:22

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Published on: July 16, 2011

Area of Science:

  • Sports Science
  • Biochemistry
  • Analytical Chemistry

Background:

  • Erythropoiesis-stimulating agents (ESAs) are performance-enhancing drugs banned in sports.
  • The development of new ESAs necessitates continuous advancements in anti-doping detection methods.
  • The World Anti-Doping Code prohibits the use of ESAs.

Purpose of the Study:

  • To review current and emerging strategies for detecting ESA doping.
  • To highlight advancements in ESA detection methods between 2009 and 2010.
  • To discuss the adaptation of detection techniques to new ESA substances.

Main Methods:

  • Isoelectric focusing in polyacrylamide slab gels (IEF-PAGE) for urine analysis.
  • Immunoaffinity purification and ELISA for serum/plasma analysis of specific ESAs like Mircera.
  • Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for detecting other ESAs.
  • Utilizing the haematological module of the Athlete Biological Passport for indirect testing.

Main Results:

  • Detection methods have evolved from urine-based isoform profiling to include blood analysis.
  • Specific assays like Mircera ELISA and SDS-PAGE were developed to counter new ESAs.
  • The Athlete Biological Passport represents a significant advancement in indirect ESA doping detection.

Conclusions:

  • Continuous innovation in ESA detection is crucial to combat doping.
  • A multi-matrix and multi-method approach is essential for effective ESA anti-doping.
  • The Athlete Biological Passport offers a powerful tool for monitoring athletes for potential ESA abuse.