Related Experiment Video
Updated: Jun 1, 2026

A Synthetic Methodology for Preparing Impregnated and Grafted Amine-Based Silica Composites for Carbon Capture
Published on: September 29, 2023
Effect of carbon loading on the extraction properties of C-18 bonded silica used for solid-phase extraction of acidic
P Martin1, E D Morgan, I D Wilson
1Department of Safety of Medicines, Zeneca Pharmaceuticals, Mereside, Alderley Park, Macclesfield, Cheshire SK10 4TG, U.K., and Department of Chemistry, University of Keele, Staffordshire ST5 5BG, U.K.
Abstract:
A range of C-18 bonded phases for solid-phase extraction (SPE), with different carbon loadings and degrees of end capping, have been evaluated for the extraction of model acidic and basic analytes (anisic acid and propranolol, respectively). High carbon loadings (18 and 22%) were associated with poor extraction properties with losses on application and wash steps. Recovery of extracted analytes from such materials was also inefficient, suggesting poor mass transfer properties. A low-carbon-loaded phase (5%), though capable of efficient extraction of the analytes, was seen to be poorly retentive. The best SPE phases for either analyte were those of intermediate carbon loading (∼15%). Significant effects of end capping were only observed for the basic analyte propranolol, where retention was probably mediated by silanophilic interactions.
More Related Videos
Related Concept Videos
Ion-Exchange Chromatography
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Extraction: Advanced Methods
Analyte Adsorption and Distribution
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...

