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Global gene expression profile for swarming Bacillus cereus bacteria
Sara Salvetti1, Karoline Faegri, Emilia Ghelardi
1Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Università di Pisa, Via San Zeno 35-39, 56127 Pisa, Italy. sara.salvetti@for.unipi.it
Applied and Environmental Microbiology
|June 7, 2011
Summary
Bacillus cereus swarming motility involves significant gene expression changes, including increased flagellin production and altered daptomycin resistance. This collective behavior impacts cellular metabolism and colonization strategies.
Area of Science:
- Microbiology
- Bacterial Motility
- Gene Expression Analysis
Background:
- Bacillus cereus exhibits swarming motility, a collective behavior enabling surface colonization.
- Swarming involves the differentiation of cells into hyperflagellate swarm cells.
Purpose of the Study:
- To analyze the genome-wide transcriptional response of Bacillus cereus during swarming.
- To identify genes differentially expressed during swarming and their functional roles.
Main Methods:
- Genome-wide transcriptional analysis of B. cereus ATCC 14579 during swarming.
- Quantitative reverse transcription-PCR (qRT-PCR) to validate gene expression.
- Phenotypic assays to assess daptomycin susceptibility.
Main Results:
- Swarming induced differential expression of 118 genes (>2-fold change), with downregulation of basic metabolism genes.
- Genes for flagellin, K(+) transport, phage, and hemolysin BL (HBL) enterotoxin were induced.
- Upregulation of BC1435 and BC1436 (daptomycin resistance orthologs) correlated with reduced daptomycin susceptibility in swarm cells.
Conclusions:
- Bacillus cereus swarming involves coordinated transcriptional changes affecting motility, metabolism, and virulence.
- Specific genes, including the hbl operon and BC1435/BC1436, play crucial roles in swarming behavior and daptomycin resistance.
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