Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 1, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
08:59

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Published on: November 23, 2016

Capacitive Approach To Determine Phospholipase A(2) Activity toward Artificial and Natural Substrates.

V M Mirsky1, M Mass, C Krause

  • 1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany.

Analytical Chemistry
|June 8, 2011
PubMed
Summary

A novel capacitive sensor detects phospholipase A(2) activity by measuring capacitance changes. This method offers sensitive detection of enzymes using short-chain or long-chain phospholipids.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The implementation of frailty assessment in gynecologic oncology: an international multicenter JAGO-NOGGO survey.

Archives of gynecology and obstetrics·2025
Same author

The InSight HP<sup>3</sup> Penetrator (Mole) on Mars: Soil Properties Derived from the Penetration Attempts and Related Activities.

Space science reviews·2022
Same author

Dual-mode auto-calibrating resistance thermometer: A novel approach with Johnson noise thermometry.

The Review of scientific instruments·2021
Same author

Images from the surface of asteroid Ryugu show rocks similar to carbonaceous chondrite meteorites.

Science (New York, N.Y.)·2019
Same author

Hayabusa2 arrives at the carbonaceous asteroid 162173 Ryugu-A spinning top-shaped rubble pile.

Science (New York, N.Y.)·2019
Same author

[Rare differential diagnosis of PET-positive pulmonary lesions].

Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2019

Area of Science:

  • Biochemistry
  • Biosensors
  • Analytical Chemistry

Background:

  • Phospholipase A(2) (PLA(2)) plays a crucial role in various biological processes.
  • Accurate determination of PLA(2) activity is essential for research and diagnostics.
  • Existing methods for phospholipase activity detection have limitations in sensitivity or substrate specificity.

Purpose of the Study:

  • To develop a novel capacitive sensing method for determining phospholipase activity.
  • To investigate the use of self-assembled monolayers as sensing interfaces.
  • To achieve sensitive and quantitative detection of phospholipase A(2).

Main Methods:

  • Fabrication of capacitive sensors with a structure of Au/S(CH(2))(17)CH(3)/substrate/electrolyte.
  • Utilizing the hydrolysis of phospholipid substrates mediated by phospholipase A(2).

More Related Videos

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
10:31

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

A Fluorescence-based Assay of Phospholipid Scramblase Activity
09:52

A Fluorescence-based Assay of Phospholipid Scramblase Activity

Published on: September 20, 2016

Related Experiment Videos

Last Updated: Jun 1, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
08:59

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Published on: November 23, 2016

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
10:31

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

A Fluorescence-based Assay of Phospholipid Scramblase Activity
09:52

A Fluorescence-based Assay of Phospholipid Scramblase Activity

Published on: September 20, 2016

  • Monitoring the change in electrode capacitance due to the desorption of water-soluble hydrolysis products.

    • Main Results:

    • The capacitive sensor demonstrated sensitive qualitative detection of phospholipases using short-chain phospholipids.
    • A semiquantitative detection method was developed for long-chain natural substrates using β-cyclodextrin as a solubilizing agent.
    • The detection limit for phospholipase A(2) was determined to be approximately 0.5 ng/mL (500 μunits/mL).

    Conclusions:

    • A novel capacitive sensing approach provides a sensitive platform for phospholipase activity determination.
    • The method is adaptable for both qualitative and semiquantitative analysis using different substrate strategies.
    • This biosensor offers a promising tool for research involving phospholipase A(2).