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Updated: Jun 1, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Quantitative Measurements in Continuous-Flow HPLC/NMR.

M Godejohann1, A Preiss, C Mügge

  • 1Fraunhofer-Institut für Toxikologie und Aerosolforschung, Nikolai-Fuchs-Strasse 1, D-30625 Hannover, Germany.

Analytical Chemistry
|June 8, 2011
PubMed
Summary
This summary is machine-generated.

Two methods for quantitative determination in continuous-flow HPLC/NMR were developed. One method involves mixing an internal standard into the mobile phase, while the other injects it during the run, showing good accuracy for explosives analysis.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Chromatography

Background:

  • Quantitative determination of compounds is crucial in various scientific fields.
  • Continuous-flow High-Performance Liquid Chromatography/Nuclear Magnetic Resonance (HPLC/NMR) offers powerful analytical capabilities.
  • Accurate quantification requires robust methodologies, especially for complex samples like explosives.

Purpose of the Study:

  • To describe and validate two distinct methods for quantitative determination using continuous-flow HPLC/NMR.
  • To assess the accuracy and precision of these methods for analyzing nitroaromatic compounds and explosives.
  • To establish reliable analytical protocols for trace compound detection.

Main Methods:

  • Development of two internal standard methods for continuous-flow HPLC/NMR.
  • Method 1: Internal standard (caffeine) added to the mobile phase.
  • Method 2: Internal standard injected onto the column during the chromatographic run, validated for nitroaromatic compounds and explosives.

Main Results:

  • Both methods provide quantitative determination of compounds in continuous-flow HPLC/NMR.
  • Method 2, validated with nitroaromatic compounds and explosives, showed deviations below 10%.
  • Relative standard deviation ranged from 2% at microgram levels to 40% at the limit of detection.

Conclusions:

  • The described internal standard methods are effective for quantitative analysis in continuous-flow HPLC/NMR.
  • The injection method offers reliable quantification for nitroaromatic compounds and explosives.
  • The methods provide a valuable tool for accurate compound determination, particularly in trace analysis.