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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Full sequence analysis of the original Sapporo virus
Kaori Nakanishi1, Masatoshi Tatsumi, Kazuko Kinoshita-Numata
1Department of Pediatrics, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-Ku, Sapporo 060-8543, Japan.
Microbiology and Immunology
|June 8, 2011
Summary
This study sequenced the Sapporo virus (SV82) genome, revealing its genetic makeup and evolutionary relationships. The findings provide foundational data for understanding sapovirus diversity and origins.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Sapoviruses are a significant cause of human gastroenteritis.
- The genetic diversity of sapoviruses necessitates comprehensive genomic characterization.
- Sapporo virus (SV82) serves as the prototype strain for this genus.
Purpose of the Study:
- To determine the full-length genome sequence of the Sapporo virus (SV82) prototype.
- To analyze the genetic structure and evolutionary relationships of Sapporo virus.
- To contribute foundational genomic data for sapovirus research.
Main Methods:
- RNA extraction from fecal samples.
- Reverse transcription polymerase chain reaction (RT-PCR) for amplification.
- Direct sequencing and bioinformatic analysis of PCR products.
Main Results:
- The complete genome sequence of Sapporo virus (SV82) was identified, comprising 7433 nucleotides.
- Analysis revealed three open reading frames within the viral genome.
- The Sapporo strain exhibited 91.7% nucleotide sequence identity to the Manchester virus.
- Phylogenetic analysis confirmed the close relationship of Sapporo virus to other genogroup I sapoviruses.
Conclusions:
- The full genome sequence provides essential data for Sapporo virus classification.
- Phylogenetic placement clarifies the evolutionary history within the Sapovirus genus.
- This study enhances our understanding of sapovirus genetic diversity and evolution.
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