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Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...

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Related Experiment Video

Updated: Jun 1, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Peer Reviewed: Understanding Reversed-Phase LC with solid-state NMR.

M Pursch, L C Sander, K Albert

    Analytical Chemistry
    |June 9, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Nuclear Magnetic Resonance (NMR) techniques provide versatile tools for analyzing bonded-phase structure, surface chemistry, and dynamic behaviors. These methods are crucial for understanding material properties and interactions.

    Related Experiment Videos

    Last Updated: Jun 1, 2026

    Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
    14:55

    Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

    Published on: September 17, 2017

    Area of Science:

    • Analytical Chemistry
    • Materials Science
    • Physical Chemistry

    Background:

    • Understanding the properties of bonded phases is critical in various scientific applications.
    • Characterizing surface chemistry and stability influences material performance.
    • Dynamic behavior studies are essential for predicting material longevity and function.

    Purpose of the Study:

    • To highlight the utility of Nuclear Magnetic Resonance (NMR) techniques.
    • To demonstrate NMR's application in analyzing bonded-phase structure.
    • To showcase NMR's role in investigating surface chemistry, stability, and dynamic behavior.

    Main Methods:

    • Utilizing various Nuclear Magnetic Resonance (NMR) spectroscopy methods.
    • Applying NMR to probe the structural characteristics of bonded phases.
    • Employing NMR to assess surface chemical properties and material stability.

    Main Results:

    • NMR techniques effectively elucidate bonded-phase structure.
    • Surface chemistry and stability can be accurately determined using NMR.
    • Dynamic behaviors of materials are successfully studied via NMR.

    Conclusions:

    • NMR spectroscopy is a powerful and versatile tool for comprehensive material characterization.
    • The application of NMR extends to understanding complex surface phenomena and material dynamics.
    • NMR provides invaluable insights for advancing materials science and analytical chemistry.