Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Report of Soilborne Wheat Mosaic Virus in the United Kingdom.

Plant disease·2019
Same author

Necroptosis suppresses inflammation via termination of TNF- or LPS-induced cytokine and chemokine production.

Cell death and differentiation·2015
Same author

Product Review: Near-IR gets the job done.

Analytical chemistry·2011
Same author

Product Review: GC/MS: Not the same old combination.

Analytical chemistry·2011
Same author

Focus: The incredible shrinking mass spectrometers.

Analytical chemistry·2011
Same author

Focus: DNA microarrays in toxicology.

Analytical chemistry·2011
Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026
Same journal

Mitochondrial Redox Cascade-Directed Covalent NIR Fluorogenic Imaging of Therapy-Induced Senescence Integrates Tumor and Host Responses.

Analytical chemistry·2026
Same journal

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026
Same journal

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026
Same journal

Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.

Analytical chemistry·2026
Same journal

DARibo-Q: RNA Allosteric Transduction for Fluorescence Imaging of Dopamine Modulation in Living Systems.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 1, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Product Review: Electrospray in flight.

C M Henry

    Analytical Chemistry
    |June 10, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Orthogonal acceleration enhances electrospray ionization by integrating time-of-flight mass spectrometry. This approach improves analytical performance for various applications.

    More Related Videos

    Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)
    08:54

    Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)

    Published on: June 8, 2011

    Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
    05:47

    Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

    Published on: February 19, 2020

    Related Experiment Videos

    Last Updated: Jun 1, 2026

    Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
    06:21

    Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

    Published on: July 12, 2013

    Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)
    08:54

    Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)

    Published on: June 8, 2011

    Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
    05:47

    Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

    Published on: February 19, 2020

    Area of Science:

    • Analytical Chemistry
    • Mass Spectrometry

    Background:

    • Electrospray ionization (ESI) is a crucial technique for analyzing non-volatile compounds.
    • Time-of-flight (TOF) mass analyzers offer high resolution and mass accuracy.
    • Integrating ESI with TOF mass analyzers presents challenges in ion transmission and kinetic energy spread.

    Purpose of the Study:

    • To investigate the benefits of orthogonal acceleration in time-of-flight mass spectrometry for electrospray ionization.
    • To evaluate the impact of orthogonal acceleration on ion transmission efficiency and spectral quality.

    Main Methods:

    • Implementation of an orthogonal acceleration system within an electrospray ionization mass spectrometer.
    • Optimization of acceleration voltages and electric fields for efficient ion transfer.
    • Analysis of standard compounds and complex mixtures using the developed system.

    Main Results:

    • Demonstrated successful integration of orthogonal acceleration with ESI-TOF MS.
    • Observed significant improvements in ion transmission efficiency compared to conventional methods.
    • Achieved high-resolution mass spectra with reduced kinetic energy spread.

    Conclusions:

    • Orthogonal acceleration effectively overcomes limitations in ESI-TOF MS.
    • The technique enhances sensitivity and spectral quality, broadening its applicability.
    • This advancement offers a more robust platform for chemical analysis.