Related Experiment Video
Updated: Jun 1, 2026

Embryo Microinjection for Transgenesis in Drosophila
Published on: June 7, 2024
Successive and targeted DNA integrations in the Drosophila genome by Bxb1 and phiC31 integrases
Juan Huang1, Pallavi Ghosh, Graham F Hatfull
1Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.
Abstract:
At present ϕC31 is the only phage integrase system available for directionally regulated site-specific DNA integration in the Drosophila genome. Here we report that mycobacteriophage Bxb1 integrase also mediates targeted DNA integration in Drosophila with high specificity and efficiency. By alternately using Bxb1 and ϕC31, we were able to carry out multiple rounds of successive and targeted DNA integrations in our genomic engineering founder lines for the purpose of generating complex knock-in alleles.
Related Concept Videos
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Polytene Chromosomes
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...

