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Isolation and characterization of microsatellite loci in Schoenoplectus juncoides (Cyperaceae)1
1Laboratory of Plant Resource Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 74 Kitainayazuma, Seika-cho, Kyoto 619-0244, Japan. ohsako@kpu.ac.jp
Premise Of The Study:
We developed microsatellite markers for Schoenoplectus juncoides, a weed found in rice paddy fields in eastern Asia.
Methods And Results:
Ten dinucleotide microsatellite loci were isolated, and genetic variability within and among local populations were characterized. The number of alleles per locus ranged from 2 to 6 with a mean of 3.6. Expected heterozygosity per locus ranged from 0.067 to 0.471, whereas observed heterozygosity showed much lower values ranging from 0.000 to 0.083. Most of the loci showed significant deviation from Hardy-Weinberg equilibrium and high inbreeding coefficient values (0.525-1.000).
Conclusions:
All the 10 microsatellite loci displayed polymorphism within and among populations. These loci are useful genetic markers for microevolution and ecological studies of S. juncoides populations.
