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Related Experiment Video

Updated: Jun 1, 2026

Stable Isotope In-Vivo Labeling for Mass-Spectrometry Identification of Paternal Metabolites Transferred from Sperm to Oocyte During Fertilization

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Published on: June 17, 2025

Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect.

Takashi Kohda¹, Narumi Ogonuki, Kimiko Inoue

¹Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.

Biochemical and Biophysical Research Communications

|June 11, 2011

Summary

Intracytoplasmic sperm injection (ICSI) causes temporary transcriptome changes in neonatal mice, but no lifelong or transgenerational effects were observed. Further evaluation of neonatal period effects is needed.

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Published on: December 28, 2021

Area of Science:

Reproductive Medicine
Developmental Biology
Genomics

Background:

Faithful transcriptome regulation is vital for development and assisted reproductive technologies (ART).
Concerns persist regarding the developmental impact of Intracytoplastoplasmic Sperm Injection (ICSI) despite numerous studies.
A clear consensus on ICSI's long-term effects on children remains elusive.

Purpose of the Study:

To assess the pre- and postnatal effects of ICSI on transcriptome and phenotype in a mouse model.
To investigate the persistence and potential transgenerational transmission of ICSI-induced changes.

Main Methods:

Comprehensive transcriptome and phenotypic analyses were performed on mice under controlled conditions.
Comparison between ICSI and in vitro fertilization (IVF) groups.
Assessment of gene expression and phenotypic profiles in adult offspring and subsequent generations.

Main Results:

ICSI, unlike IVF, induced distinct, long-lasting transcriptome changes persisting into the neonatal stage.
No significant differences in gene expression or phenotypic profiles were observed in adult ICSI mice.
No evidence of ICSI effects being transmitted to the next generation through natural mating.

Conclusions:

ICSI does not appear to cause lifelong or transgenerational effects in the studied mouse model.
Distinct transcriptome alterations occur during the neonatal period following ICSI.
Further research is warranted to fully evaluate the specific effects of ICSI during the neonatal period.