DNA collection from used toothbrushes as a means to decedent identification

Comparison of DNA profiles is often used in verifying the identification of deceased human beings when other easier, quicker, and less expensive means to identification are not possible. Fifty-five adult subjects divided into 3 groups provided a used toothbrush along with a small bloodstain control for DNA analysis and comparison. Results indicate that there is no significant difference in the quantity and quality of DNA recovered from a toothbrush that has been used for 1 month versus 3 months versus random periods. The results of this study confirm earlier conclusions that a used toothbrush is a reliable source of antemortem DNA from a putative decedent. The use of aviation snips to remove a small portion of the toothbrush head provides an easy, inexpensive method of obtaining a sample for DNA extraction. The authors recommend this method as a standardized technique for use in forensic DNA laboratories.