Related Experiment Videos
Screening for tuberculosis and its histological pattern in patients with enlarged lymph node.
Hussain Gad Elkarim Ahmed1, Akram Saleh Nassar, Ibrahim Ginawi
1Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.
Pathology Research International
|June 11, 2011
Summary
Histopathology is the most reliable method for diagnosing tuberculous lymphadenitis, as Ziehl-Neelsen (ZN) and fluorescent stains showed low sensitivity in identifying Mycobacterium tuberculosis in Yemeni lymph node biopsies.
Area of Science:
- Medical Microbiology
- Pathology
- Infectious Diseases
Background:
- Tuberculosis (TB) is a significant global health issue, with tuberculous lymphadenitis being a common extrapulmonary manifestation.
- Yemen faces a substantial burden of tuberculosis, necessitating accurate diagnostic methods for lymph node involvement.
Purpose of the Study:
- To investigate the histopathological patterns of tuberculous lymphadenitis in Yemen.
- To evaluate the diagnostic reliability of Ziehl-Neelsen (ZN) and fluorescent staining methods for Mycobacterium tuberculosis identification in lymph node biopsies.
Main Methods:
- A retrospective analysis of 100 lymph node biopsies previously diagnosed with tuberculous lymphadenitis.
- Tissue sections were stained using Hematoxylin and eosin (H&E), ZN, and fluorescent methods for microscopic examination.
Main Results:
- All 100 specimens confirmed the histopathological features of tuberculous lymphadenitis.
- Key histological findings included giant cells (88%), caseation (84%), and epithelioid cells (80%).
- ZN staining identified Mycobacterium tuberculosis in only 3% of cases, while fluorescent staining detected it in 9%.
Conclusions:
- Conventional ZN and fluorescent staining methods exhibit low sensitivity for diagnosing tuberculous lymphadenitis.
- Histopathology remains the gold standard for diagnosing tuberculous lymphadenitis.
- Further confirmation with sensitive methods like PCR or immunohistochemistry is recommended for suspected cases with negative staining results.
Related Concept Videos
01:26Pulmonary Tuberculosis IV
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
01:31Pulmonary Tuberculosis III
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
The first classification is based on the development of the disease, and it includes the following categories:
01:28Pulmonary Tuberculosis V
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...
01:28Pulmonary Tuberculosis II
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...