Multiplexed near-infrared in vivo imaging complementarily using quantum dots and upconverting NaYF4:Yb3+,Tm3+...

Area of Science:

• Biomedical Imaging
• Nanotechnology
• Optical Spectroscopy

Background:

• Multiplexed imaging is crucial for simultaneous analysis of multiple biological targets.
• Near-infrared (NIR) imaging offers deep tissue penetration and reduced autofluorescence.
• Existing multiplexing techniques face limitations in sensitivity and specificity.

Purpose of the Study:

• To demonstrate a novel multiplexed NIR in vivo imaging approach.
• To utilize quantum dots and NaYF(4):Yb(3+),Tm(3+) nanoparticles for enhanced imaging.
• To validate the temporal multiplexing capability for cellular imaging and trafficking studies.

Main Methods:

• Utilized a combination of quantum dots and lanthanide-based (NaYF(4):Yb(3+),Tm(3+)) nanoparticles as NIR probes.
• Implemented 'temporal' multiplexing by alternating excitation wavelengths.
• Developed signal processing algorithms to unmix emissions from different probes.

Main Results:

• Successfully demonstrated multiplexed NIR in vivo imaging in animal models.
• Achieved distinct unmixing of signals from different nanoparticle probes.
• Visualized cellular trafficking and distribution in vivo using the developed technique.

Conclusions:

• The proposed method enables robust multiplexed NIR in vivo imaging.
• Temporal multiplexing offers a viable strategy for simultaneous detection of multiple targets.
• This technology holds promise for advancing preclinical research and diagnostics.