Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent advances in protein engineering and synthetic applications of amino acid oxidases.

Bioresource technology·2026
Same author

A robust multi-criteria supplier selection framework based on linguistic cubic interval-valued intuitionistic fuzzy aggregation operators.

Scientific reports·2026
Same author

Defect Diamond-Like d<sup>10</sup> Metal Indium Selenium With Strong Second-Harmonic Generation and Enhanced Laser-Induced Damage Threshold.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Cerium-coated Fe<sub>3</sub>O<sub>4</sub> nanocomposite enhances salt stress tolerance in maize by modulating photosynthetic efficiency, antioxidant defense, and cellular ultrastructure.

Frontiers in plant science·2026
Same author

Role of UAVs in precision pesticide spraying and environmental safety by combating pesticide resistance and safety of natural enemies.

Pest management science·2026
Same author

Genome based characterization and metabolic pathway elucidation of dibenzothiophene desulfurization in the novel sulfur metabolizing <i>Mycolicibacterium</i> sp. J2.

3 Biotech·2026
Same journal

Current Status of PSMA-Targeted Agents Based on Small Molecules for Prostate Cancer in Preclinical Studies.

Nuclear medicine and molecular imaging·2026
Same journal

Fibroblast Activation Protein-Targeted SPECT Imaging Using <sup>99m</sup>Tc: A Comprehensive Review.

Nuclear medicine and molecular imaging·2026
Same journal

The 2026 Special Issue on Radiopharmaceuticals: from Molecular Design to Clinical Translation.

Nuclear medicine and molecular imaging·2026
Same journal

Feasibility of [<sup>99m</sup>Tc]Tc-Mannosylated Human Serum Albumin for Lymphoscintigraphy: A Phase 1/2 Clinical Trial.

Nuclear medicine and molecular imaging·2026
Same journal

Automated Synthesis of Radiopharmaceuticals: Technology, Applications, and Regulatory Perspectives.

Nuclear medicine and molecular imaging·2026
Same journal

Development of Antibody and Antibody Fragment-Based Radiopharmaceuticals for Cancer Imaging and Therapy.

Nuclear medicine and molecular imaging·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2026

Imaging CD19+ B Cells in an Experimental Autoimmune Encephalomyelitis Mouse Model using Positron Emission Tomography
09:41

Imaging CD19+ B Cells in an Experimental Autoimmune Encephalomyelitis Mouse Model using Positron Emission Tomography

Published on: January 20, 2023

Aggregation Issues in Radioactive Copper-Labeled Antibodies.

Muhammad Zeeshan1, Huu Bao Nguyen2, Jeongsoo Yoo1

  • 1Department of Molecular Medicine, BK21 FOUR KNU Convergence Educational Program, School of Medicine, Kyungpook National University, Daegu, 41944 Republic of Korea.

Nuclear Medicine and Molecular Imaging
|June 11, 2026
PubMed
Summary
This summary is machine-generated.

Copper radioisotopes like Cu-64 are vital for precision oncology imaging and therapy. This review highlights strategies to control antibody aggregation, a critical quality control issue for radioimmunoconjugates, emphasizing dynamic light scattering for detection.

Keywords:
AggregationCopper-64Dynamic light scatteringMonoclonal antibodiesQuality controlRadioimmunoconjugates

More Related Videos

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
11:34

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting
11:58

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting

Published on: March 8, 2018

Related Experiment Videos

Last Updated: Jun 12, 2026

Imaging CD19+ B Cells in an Experimental Autoimmune Encephalomyelitis Mouse Model using Positron Emission Tomography
09:41

Imaging CD19+ B Cells in an Experimental Autoimmune Encephalomyelitis Mouse Model using Positron Emission Tomography

Published on: January 20, 2023

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
11:34

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting
11:58

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting

Published on: March 8, 2018

Area of Science:

  • Radiopharmaceutical chemistry
  • Precision oncology
  • Molecular imaging and therapy

Background:

  • Radioimmunoconjugates combine radionuclides with monoclonal antibodies (mAbs) for targeted oncology applications.
  • Copper radioisotopes, particularly Cu-64, offer versatile properties for diagnostic and therapeutic uses in immuno-PET and theranostics.
  • Antibody radiolabeling faces quality control challenges, notably protein aggregation, impacting product efficacy.

Purpose of the Study:

  • To review the significance of copper radioisotopes in radioimmunoconjugates.
  • To discuss strategies for purifying immunoconjugates for clinical use.
  • To address the critical issue of antibody aggregation in radiolabeled products.

Main Methods:

  • Literature review of copper radioisotopes, purification strategies, and aggregation in radioimmunoconjugates.
  • Evaluation of various methods for detecting antibody aggregation.
  • Focus on dynamic light scattering (DLS) as a sensitive quality control tool.

Main Results:

  • Cu-64 is a promising radionuclide for immuno-PET and theranostic applications.
  • Antibody aggregation negatively impacts pharmacokinetics and immunogenicity.
  • Dynamic light scattering (DLS) offers superior sensitivity for detecting hidden aggregates compared to traditional methods.
  • Hidden aggregates identified by DLS inversely correlate with tumor uptake.

Conclusions:

  • Effective purification and aggregation control are essential for clinically acceptable radioimmunoconjugates.
  • Dynamic light scattering (DLS) is a valuable orthogonal quality control method for assessing antibody aggregation.
  • Minimizing aggregation is crucial for optimizing the performance and safety of radiolabeled antibodies in oncology.