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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...

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Related Experiment Video

Updated: Jun 1, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

How the atomic force microscope works?

Bruno Torre1, Davide Ricci, Pier Carlo Braga

  • 1Robotics, Brain and Cognitive Sciences Department, Italian Institute of Technology, Genoa, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2011
PubMed
Summary
This summary is machine-generated.

This chapter introduces the atomic force microscope (AFM), detailing its working principles, technical implementations, and recent advancements. It compares AFM with scanning electron microscope (SEM) and transmission electron microscope (TEM) for diverse applications.

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Last Updated: Jun 1, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
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10:15

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Published on: July 22, 2015

Area of Science:

  • Surface science and nanotechnology
  • Microscopy techniques

Background:

  • Atomic Force Microscopy (AFM) offers high-resolution surface imaging.
  • Understanding AFM principles is crucial for various scientific disciplines.

Purpose of the Study:

  • To provide a concise introduction to AFM working principles and technical aspects.
  • To serve as a foundational guide for new users and experienced researchers.

Main Methods:

  • Overview of AFM working principles and common technical implementations.
  • Comparison of AFM with Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM).
  • Discussion of different instrument architectures and operational suggestions for air and liquid environments.

Main Results:

  • Highlights recent technical improvements in AFM.
  • Compares AFM capabilities, sample interactions, and applications with SEM and TEM.
  • Provides practical guidance for initiating AFM experiments.

Conclusions:

  • AFM is a versatile tool with evolving capabilities.
  • Understanding AFM fundamentals aids in selecting appropriate instrumentation and optimizing experimental outcomes.
  • Future developments in AFM techniques are outlined.