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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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Related Experiment Video

Updated: Jun 1, 2026

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
10:15

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Published on: July 22, 2015

Imaging and interrogating native membrane proteins using the atomic force microscope.

Andreas Engel1

  • 1Maurice E. Müller Institute for Structural Biology, Biozentrum, University of Basel, Basel, Switzerland. andreas.engel@unibas.ch

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2011
PubMed
Summary
This summary is machine-generated.

Atomic force microscopy (AFM) images membrane proteins in their native state without detergents. This powerful technique allows observation of biological molecular machines and quantitative analysis of their function.

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Last Updated: Jun 1, 2026

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
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Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
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Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • Membrane proteins are crucial for cellular functions, residing within lipid bilayers.
  • Detergents are traditionally used to extract and study membrane proteins, but can alter their native structure.
  • Atomic force microscopy (AFM) offers a non-disruptive method for studying membrane proteins.

Purpose of the Study:

  • To highlight the capabilities of AFM for imaging and manipulating membrane proteins in their native environment.
  • To discuss advancements in AFM technology for analyzing membrane protein function.
  • To explore the potential of AFM in conjunction with single-molecule force spectroscopy.

Main Methods:

  • Adsorbing membranes to flat solid supports for raster-scanning with an atomically sharp AFM tip.
  • Utilizing physiological solutions to maintain the native state of membrane proteins during imaging.
  • Developing multifunctional AFM probes for simultaneous topographic and electrical property measurements.

Main Results:

  • AFM enables high-resolution imaging of native membranes and their embedded proteins.
  • Quantitative interpretation of AFM data provides insights into molecular machine operation.
  • Multifunctional probes allow for simultaneous acquisition of topographical and electrical data.

Conclusions:

  • AFM is a powerful tool for observing and analyzing membrane proteins in their native state.
  • Advancements in AFM technology enhance the study of biological molecular machines.
  • Future applications of AFM and single-molecule force spectroscopy hold significant promise for membrane protein research.