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Updated: Jun 1, 2026

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
Published on: September 7, 2009
Determination of the kinetic on- and off-rate of single virus-cell interactions
Christian Rankl1, Linda Wildling, Isabel Neundlinger
1Institute for Biophysics, University of Linz, Linz, Austria.
Abstract:
Human rhinoviruses are the causative agents of the common cold. The serotypes belonging to the minor receptor group attach to members of the low-density lipoprotein receptor family and enter the host cell via receptor-mediated endocytosis. Receptor binding, the very first step in infection, was characterized by force spectroscopy measurements at the single molecule level. We demonstrate how kinetic on- and off-rate constants can be derived from such experiments carried out with the atomic force microscope.
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