Related Experiment Video
Updated: Jun 1, 2026
07:58
Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
High-Speed TOFMS Detection for Capillary Electrophoresis
I M Lazar1, A L Rockwood, E D Lee
1Sensar Corporation, 1662 West 820 North, Provo, Utah 84601, and Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602.
Analytical Chemistry
|June 14, 2011
Summary
A new data acquisition system enhances capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) speed. This system achieves high spectral storage rates, enabling sensitive detection in the low attomole range.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Separation Science
Background:
- Capillary electrophoresis (CE) coupled with time-of-flight mass spectrometry (TOFMS) is a powerful analytical technique.
- High spectral acquisition rates are crucial for comprehensive analysis of complex samples.
- Limitations in data storage rates can hinder the performance of high-speed CE-TOFMS systems.
Purpose of the Study:
- To evaluate a novel, high-speed data acquisition system for CE-TOFMS.
- To assess the system's capability for high spectral storage rates.
- To determine the detection limits achievable with the new system.
Main Methods:
- Implementation of a new high-speed data acquisition system.
- Coupling the system with capillary electrophoresis (CE).
- Time-of-flight mass spectrometry (TOFMS) detection was employed.
- Continuous infusion investigations were performed.
Main Results:
- The data acquisition system supported high spectral acquisition rates of 4 kHz.
- A spectral storage rate of 80 spectra per second was successfully achieved.
- Low attomole (amol) detection limits, specifically 10-25 amol, were obtained.
Conclusions:
- The developed high-speed data acquisition system significantly improves CE-TOFMS performance.
- The system enables high-throughput analysis with excellent sensitivity.
- This advancement is valuable for applications requiring rapid and sensitive mass spectrometry detection.
Related Concept Videos
01:20Electrophoresis: Overview
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
01:20Capillary Electrophoresis: Instrumentation
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
01:30Capillary Electrophoresis: Applications
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
01:15High-Performance Liquid Chromatography: Types of Detectors
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
01:21Tandem Mass Spectrometry
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
01:25Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...