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Related Concept Videos

Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

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Updated: Jun 1, 2026

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
05:48

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry

Published on: September 5, 2014

Fragment-Free Mass Spectrometric Analysis with Jet Cooling/VUV Photoionization.

E Nir1, H E Hunziker, M S de Vries

  • 1Department of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

Analytical Chemistry
|June 14, 2011
PubMed
Summary
This summary is machine-generated.

We developed a method using laser desorption, jet cooling, and VUV single-photon photoionization to achieve fragment-free mass spectra for large molecules, aiding mixture analysis.

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Last Updated: Jun 1, 2026

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
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Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Spectroscopy

Background:

  • Accurate mass determination of large molecules is crucial for analyzing complex mixtures.
  • Traditional mass spectrometry methods often lead to fragmentation, obscuring parent molecular information.
  • Selective ionization techniques are needed to overcome fragmentation challenges.

Purpose of the Study:

  • To demonstrate a novel method for obtaining fragment-free mass spectra of large molecules.
  • To explore the potential for controlled fragmentation to gain structural insights.
  • To enhance selectivity in analyzing hydrocarbon mixtures.

Main Methods:

  • Utilizing laser desorption for sample introduction.
  • Employing jet cooling to minimize molecular internal energy.
  • Implementing vacuum ultraviolet (VUV) single-photon photoionization for ionization.
  • Varying cooling conditions and VUV wavelengths for controlled fragmentation and selectivity.

Main Results:

  • Successfully obtained fragment-free mass spectra for large molecules.
  • Demonstrated the capability to determine parent molecular masses accurately, vital for mixture analysis.
  • Achieved partial fragmentation by adjusting cooling conditions for additional structural information.
  • Showcased wavelength-dependent selectivity between aromatic and aliphatic compounds.

Conclusions:

  • The combined technique of laser desorption, jet cooling, and VUV single-photon photoionization enables high-quality mass spectra of large molecules.
  • This method is particularly valuable for analyzing complex mixtures like saturated hydrocarbons.
  • Tunable fragmentation and wavelength selectivity offer advanced analytical capabilities for structural elucidation.