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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
Published on: May 5, 2016
Real-Time Imaging through Optical Fiber Array-Assisted Laser-Induced Fluorescence of Capillary Electrophoretic
T Johansson1, M Petersson, J Johansson
1Technical Analytical Chemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden, and Division of Atomic Physics, Lund Institute of Technology, P.O. Box 118, SE-221 00 Lund, Sweden.
Analytical Chemistry
|June 14, 2011
Summary
A novel laser-induced fluorescence imaging system for capillary electrophoresis (CE) enhances detection. This advanced optical fiber array setup reveals dynamic events and improves signal-to-noise ratio for sensitive enantiomer separations.
Area of Science:
- Analytical Chemistry
- Separation Science
- Spectroscopy
Background:
- Capillary electrophoresis (CE) is a powerful separation technique.
- Laser-induced fluorescence (LIF) imaging offers sensitive detection in CE.
- Current imaging systems can be bulky and may miss dynamic separation events.
Purpose of the Study:
- To develop an advanced, compact LIF imaging detection system for CE.
- To investigate dynamic events during capillary electrophoresis separations.
- To improve the signal-to-noise ratio (S/N) for enhanced detection sensitivity.
Main Methods:
- Construction of an optical fiber array for fluorescence collection and transport.
- Integration of the fiber array with a charge-coupled device (CCD) camera.
- Evaluation using enantiomer separations of dansylated amino acids and dichlorofluorescein.
Main Results:
- The optical fiber array system is compact and captures dynamic sample migration.
- An enhanced signal-to-noise ratio (S/N) was achieved, 10 times higher than lens systems.
- A concentration limit of detection (CLOD) of 35 pM was obtained after computer postprocessing.
Conclusions:
- The developed LIF imaging system provides a compact and sensitive detection method for CE.
- The system enables the study of previously unrevealed dynamic separation phenomena.
- Advanced signal processing significantly improves detection limits, enabling zeptomole-level quantification.
