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Updated: Jun 1, 2026

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Published on: June 13, 2021

High-performance membrane chromatography of small molecules.

A Podgornik¹, M Barut, J Jančar

¹BIA Separations d.o.o., Teslova 30, SI-1000 Ljubljana, Slovenia.

Analytical Chemistry

|June 14, 2011

Summary

High-performance membrane chromatography (HPMC) effectively separates small molecules like peptides and oligonucleotides. This study demonstrates HPMC

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Area of Science:

Biochemistry
Analytical Chemistry
Separation Science

Background:

High-performance membrane chromatography (HPMC) is established for protein and plasmid DNA separations.
Limited research exists on HPMC applications for small molecule purification.

Purpose of the Study:

To explore gradient and isocratic HPMC for small molecule separation using various chemistries.
To elucidate the separation mechanisms involved in HPMC of small molecules.

Main Methods:

Utilized Convective Interaction Media (CIM) disks with different surface chemistries.
Investigated both gradient and isocratic HPMC conditions.
Analyzed separation efficiency and resolution for various small molecules.

Main Results:

Achieved efficient separation of oligonucleotides and peptides via ion-exchange HPMC.
Successfully separated small hydrophobic molecules using reversed-phase HPMC.
Demonstrated comparable peak resolution in both gradient and isocratic HPMC modes.

Conclusions:

HPMC is a versatile technique applicable to diverse small molecule separations.
Ion-exchange and reversed-phase modes are effective for specific small molecule classes.
Gradient and isocratic HPMC offer similar resolution, providing flexibility in method development.