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Updated: Jun 1, 2026
Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein
Published on: January 28, 2013
Performance of the CRAFTi portable fluorometer comparing with the HPLC method for determining serum retinol
Laksana Chaimongkol1, Siwaporn Pinkaew, Harold C Furr
1Institute of Nutrition, Mahidol University, Nakhon Pathom 73170, Thailand.
Objective:
To determine the performance of a portable fluorometer for measuring serum retinol (SR) concentration.
Design And Methods:
Serum samples were obtained from 75 factory worker women and 143 school children. SR concentration was quantified using a portable fluorometer ('CRAFTi') and HPLC analysis.
Results:
SR by HPLC (1.23 ± 0.43 μmol/L) and CRAFTi (1.16 ± 0.46 μmol/L) was significantly correlated. Sensitivity and specificity were 85.3% and 78.0% (cutoff of 1.05 μmol/L). Kappa statistics showed moderate agreement.
Conclusions:
CRAFTi portable fluorometer is a promising field-friendly tool for screening vitamin A deficiency.