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Updated: Jun 1, 2026

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores
Published on: April 15, 2019
Protein profile of Bacillus subtilis spore
Langyong Mao1, Shantong Jiang, Bin Wang
1Institute of Life Sciences, Jiangsu University, #301 Xuefu Road, Zhenjiang, Jiangsu, People's Republic of China.
Abstract:
Natural wild-type strains of Bacillus subtilis spore is regarded as a non-pathogenic for both human and animal, and has been classified as a novel food which is currently being used as probiotics added in the consumption. To identify B. subtilis spore proteins, we have accomplished a preliminary proteomic analysis of B. subtilis spore, with a combination of two-dimensional electrophoretic separations and matrix-assisted laser desorption ionization tandem time of flight mass spectrometry (MALDI-TOF-MS). In this article, we presented a reference map of 158 B. subtilis spore proteins with an isoelectric point (pI) between 4 and 7. Followed by mass spectrometry (MS) analysis, we identified 71 B. subtilis spore proteins with high level of confidence. Database searches, combined with hydropathy analysis and GO analysis revealed that most of the B. subtilis spore proteins were hydrophilic proteins related to catalytic function. These results should accelerate efforts to understand the resistance of spore to harsh conditions.
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