Related Experiment Video
Updated: Jun 1, 2026

Surgical Techniques for Catheter Placement and 5/6 Nephrectomy in Murine Models of Peritoneal Dialysis
Published on: July 19, 2018
N-Acetylcysteine protects the peritoneum from the injury induced by hypertonic dialysis solution
Déborah S S Bui1, Antônio C Seguro, Maria H Shimitzu
1Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Background:
Oxidative stress has been implicated in the development of peritoneal damage. The aim of this study was to evaluate the effects of N-acetylcysteine (NAC) in a rat peritoneal infusion model.
Methods:
Eighteen male Wistar rats were divided in 3 groups: (i) control group; (ii) HDS group, receiving peritoneal dialysis solution (PDS); and (iii) HDS+NAC group, receiving PDS and oral NAC. Six weeks later they were evaluated for dialysate to plasma urea ratio (D/P), ratio of glucose concentration in peritoneal fluid (G1/G0), thiobarbituric acid reactive substances in plasma and urine and histology of peritoneal membrane.
Results:
The HDS+NAC group presented a lower increase in solute transport (D/P 0.51 ± 0.1, and G1/GO 0.35 ± 0.06) in comparison with the HDS group (D/P 0.67 ± 0.1; p=0.03, and G1/G0 0.27 ± 0.07; p=0.01). The HDS+NAC group showed lower thiobarbituric acid reactive substance concentrations compared with the HDS group. In the treated group, the peritoneal membrane presented lower thickness.
Conclusions:
Functional and histological peritoneal changes were significantly reduced by the treatment with NAC.
Related Concept Videos
Acute Kidney Injury V: Interprofessional Care
Peritoneal Dialysis I: Introduction and Procedure
Peritoneal Dialysis II: Peritoneal Dialysis Systems and Complications
Acute Kidney Injury IV: Diagnostic Studies and Prevention
Dialysis
Acute kidney injury develops suddenly and can be caused by pre-renal causes (e.g., hypovolemia, shock), intrinsic renal causes (e.g., acute tubular necrosis), or post-renal causes (e.g., urinary obstruction). In contrast, chronic renal failure progresses gradually over time and is often...
Dialysis
