Related Experiment Video
Updated: Jun 1, 2026
09:45
Modification and Functionalization of the Guanidine Group by Tailor-made Precursors
Published on: April 27, 2017
Nanostructured functional co-polymers bioconjugate integrin inhibitors.
Aldo Laganà1, Iole Venditti, Ilaria Fratoddi
1Department of Chemistry, University of Rome Sapienza, P.le A. Moro 5, I-00185 Rome, Italy.
Journal of Colloid and Interface Science
|June 15, 2011
Summary
Functionalized polymeric nanoparticles (PNs) were synthesized and conjugated with anti-Integrin αV CD51 (aI) antibodies. These bioconjugates demonstrated reduced cell migration, showing potential for targeted drug delivery applications.
Area of Science:
- Bioconjugation chemistry
- Nanotechnology
- Materials Science
Background:
- Development of targeted drug delivery systems requires effective bioconjugation strategies.
- Polymeric nanoparticles (PNs) offer tunable properties for drug delivery applications.
- Antibody-functionalized nanoparticles can enhance targeting specificity.
Purpose of the Study:
- To synthesize and characterize bioconjugates of functionalized polymeric nanoparticles (PNs) and anti-Integrin αV CD51 (aI) antibody.
- To investigate the immobilization strategy and loading capacity of PNs with antibodies.
- To evaluate the biological efficacy of the resulting bioconjugates.
Main Methods:
- Emulsion synthesis of functionalized PNs (P(MMA-co-AA), P(MMA-co-DMPA), P(MMA-co-AM)).
- Characterization using spectroscopic techniques and scanning electron microscopy (SEM).
- Antibody immobilization and loading analysis via 1D-PAGE, MALDI-TOF, and LC/ESI-MS/MS.
Main Results:
- Successfully synthesized and characterized functionalized PNs with acid, amine, or thiol groups.
- Developed a straightforward immobilization strategy for anti-Integrin αV CD51 antibody onto PNs.
- Demonstrated reduced migration potential in anti-Integrin αV CD51 antibody-treated human kidney cells (HEK293), confirming biological efficacy.
Conclusions:
- Functionalized polymeric nanoparticles provide a versatile platform for antibody bioconjugation.
- The developed bioconjugates exhibit promising biological activity for targeted delivery applications.
- The facile immobilization and high capacity of PNs suggest broad applicability in nanomedicine.
Related Concept Videos
01:10Integrins
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
01:15Activation of Integrins
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
01:24Site-Targeted Drug Delivery Systems: Polymeric Carriers
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
01:17Intracellular Signaling Affects Focal Adhesions
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
01:31Immunoglobulin-like Cell Adhesion Molecules
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
02:04Protein-protein Interfaces
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...