Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Different transactivation processes in two bovine leukemia virus producing cell lines].

H J Wagner1, P Blankenstein, A Bondzio

  • 1Sektion Tierproduktion und Veterinärmedizin der Humboldt-Universität zu Berlin, Wissenschafts-bereich Biochemie und Lehrstuhl Virologie.

Archiv Fur Experimentelle Veterinarmedizin
|January 1, 1990
PubMed
Summary

Bovine leukemia virus (BLV) antigen synthesis and transactivation are closely correlated in cell lines. Higher BLV antigen production in FLC/BLV 44 cells correlated with increased transactivation compared to the FLC/BLV 44-4 subline.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Search for Exclusive Diphoton Production at High Mass with Tagged Protons in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2022
Same author

Odderon Exchange from Elastic Scattering Differences between pp and pp[over ¯] Data at 1.96 TeV and from pp Forward Scattering Measurements.

Physical review letters·2021
Same author

Synthetic biology as driver for the biologization of materials sciences.

Materials today. Bio·2021
Same author

[Evaluation of the fluid balance in geriatric patients-What contribution can abdominal ultrasound make?]

Zeitschrift fur Gerontologie und Geriatrie·2021
Same author

Measurement of single-diffractive dijet production in proton-proton collisions at <math> </math> with the CMS and TOTEM experiments.

The European physical journal. C, Particles and fields·2020
Same author

Diagnostic efficacy of bedside ultrasound to detect dehydration in older patients attending an emergency care unit.

Zeitschrift fur Gerontologie und Geriatrie·2020

Area of Science:

  • Veterinary Virology
  • Molecular Biology
  • Immunology

Background:

  • Bovine leukemia virus (BLV) is an oncogenic retrovirus causing significant economic losses in the cattle industry.
  • Understanding the molecular mechanisms of BLV replication and pathogenesis is crucial for developing control strategies.
  • FLC/BLV 44 and its subline FLC/BLV 44-4 represent valuable cellular models for studying BLV-related processes.

Purpose of the Study:

  • To quantitatively compare the synthesis of BLV antigens (gp51 and p24) in FLC/BLV 44 and FLC/BLV 44-4 cell lines.
  • To assess and compare the transactivation status of BLV in these two cell lines.
  • To investigate the correlation between BLV antigen synthesis and transactivation activity.

Main Methods:

  • Quantitative enzyme immunoassay (EIA) using monoclonal antibodies against BLV gp51 and p24 antigens.

Related Experiment Videos

  • Assessment of transactivation by measuring beta-galactosidase activity under the control of the BLV long terminal repeat (LTR) in transient transfection experiments.
  • Utilizing the pBLV beta Gal plasmid for reporter gene assays.

    • Main Results:

    • FLC/BLV 44 cells exhibited significantly higher synthesis of both gp51 (2-6 fold) and p24 (2 fold) compared to the FLC/BLV 44-4 subline.
    • Transient experiments revealed markedly higher beta-galactosidase activity in FLC/BLV 44 cells, indicating stronger transactivation.
    • A close correlation was observed between the intensity of BLV antigen synthesis and the level of transactivation in both cell lines.

    Conclusions:

    • The FLC/BLV 44 cell line demonstrates a higher capacity for BLV antigen production and LTR-driven transactivation compared to its subline FLC/BLV 44-4.
    • These findings highlight a strong link between BLV antigen expression levels and the virus's transactivation potential.
    • The study provides insights into the molecular regulation of BLV replication and suggests that antigen synthesis intensity can serve as an indicator of transactivation activity.